The Enzymatic Role of Lipid Metabolism in <i>Yarrowia lipolytica</i> Grown on Glycerol

More attention has been paid to the production of commercial fatty acids by microbial fermentation. The basic biochemistry lipid accumulation in oleaginous microorganisms under nitrogen limitation extensively studied. As an microorganism, Yarrowia lipolytica can produce acid-derived biofuels and biochemicals. However, response enzymes Y. glycerol remains unclear. Therefore, we aimed identify rate-limiting associated with mechanism grown on glycerol. variations key enzyme activities acid were investigated as single carbon source. source was exhausted at 10 h medium, leading substantial changes accumulation. Adenosine monophosphate deaminase activity increased immediately approximately twofold. NAD+-isocitrate dehydrogenase decreased 65% after when compared initial maximum activity. ATP citrate lyase activity, which provides substrate acetyl coenzyme A for biosynthesis, fourfold before depletion. Malic Enzyme (ME) considerably so that reducing power Nicotinamide Adenine Dinucleotide Phosphate (NADPH) required synthesis cannot be produced ME. glucose-6-phosphate from 550 nmol·min-1·mg-1-771 nmol·min-1·mg-1, suggesting main NADPH may provided pentose phosphate pathway grew medium only